bdca3 apc ms igg1 miltenyi 130 090 907 ad5 14h12 (Miltenyi Biotec)

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Miltenyi Biotec bdca3 apc ms igg1 miltenyi 130 090 907 ad5 14h12
Bdca3 Apc Ms Igg1 Miltenyi 130 090 907 Ad5 14h12, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 242 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bdca3+cd141/CD141+(BDCA-3)+Antibody%2C+anti-human/pmc11953300__41467_2025_58220_MOESM1_ESM-78-152-156
Average 94 stars, based on 242 article reviews

bdca3 apc ms igg1 miltenyi 130 090 907 ad5 14h12 - by Bioz Stars, 2026-10

94/100 stars

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Article Title: Unique CLR expression patterns on circulating and tumor-infiltrating DC subsets correlated with clinical outcome in melanoma patients
Article Snippet: In addition, BDCA1/CD1c (Beckman), BDCA3/CD141 and BDCA4/CD304 (Miltenyi Biotec) antibodies were used to depict cDC2s, cDC1s and pDCs respectively.

Marker:

Article Title: The Effect of Chronic Hepatitis B Virus Infection on BDCA3+ Dendritic Cell Frequency and Function
Article Snippet: .. For phenotypic analysis, cells were labelled with Abs recognizing CD11c (3.9), CD40 (5C3), CD45 (HI30), CD83 (HB15e), HLA-DR (LN3, all eBioscience), BDCA1/CD1c (AD5-8E7, Miltenyi Biotec), BDCA3/CD141 (AD5-14H12, Miltenyi Biotec), CD86 (2331, BD Horizon), CLEC9A (8F9, BioLegend), HBsAg (Acris), a lineage cocktail including CD3 (UCHT1, eBioscience), CD14 (61D3, eBioscience), CD19 (HIB19, eBioscience) and CD56 (MY31, BD Biosciences), and the live/dead marker Aqua (LifeTechnologies). .. Fluorescence was measured using a FACS Canto II (BD Biosciences).

Staining:

Article Title: Hepatitis B virus exploits C‐type lectin receptors to hijack cDC1s, cDC2s and pDCs
Article Snippet: .. Fresh PBMCs and LMNCs from HBV or HD/CTRL were stained with fluorochrome‐labelled anti‐human CD11c, HLA‐DR, Lineage cocktail (CD3/CD14/CD16/CD19/CD20/CD56), DNGR1/CD370/CLEC9A, CD123, MMR/CD206, DC‐SIGN/CD209 (BD Biosciences, Le Pont de Claix, France); CD45, DECTIN1/CD369/CLEC7A, CD371/CLEC12A, DEC205/CD205, Langerin/CD207, FcɛRIα; FcɣRIIA/CD32 (Biolegend, Paris, France), BDCA1/CD1c, NKp44/CD336 (Beckman Coulter, Roissy, France); BDCA2/CD303/CLEC4C, BDCA3/CD141, BDCA4/CD304 (Miltenyi Biotec, Paris, France); DCIR/CD367/CLEC4A (R&D systems, Rennes, France) and FcɣRIIA/CD32, ILT7/CD85g (eBiosiences, Paris, France) antibodies. .. Stained cells were then fixed with FACS lysing solution (BD) and further analysed using LSRII Flow Cytometer and FACSDiva software (BD).

Article Title: Hepatitis B virus exploits C‐type lectin receptors to hijack cDC1s, cDC2s and pDCs
Article Snippet: All cultures were performed in RPMI‐1640/GlutaMAX (Invitrogen, Courtaboeuf, France) supplemented with 1% non‐essential amino acids, 100 μg mL −1 gentamicin, 1 m m sodium pyruvate (Sigma), and 10% foetal bovine serum (FBS) (Invitrogen) or autologous human serum. .. Subsequently, cells were thoroughly washed with cold PBS supplemented with 2% of FBS to remove the free HBsAg particle and then, cells were stained with fluorochrome‐labelled anti‐human CD11c, HLA‐DR, Lineage cocktail, DNGR1/CD370/CLEC9A, CD123, MMR/CD206, DC‐SIGN/CD209 (BD); Lineage cocktail, CD45, DECTIN1/CD369/CLEC7A, CD371/CLEC12A, DEC205/CD205, Langerin/CD207, FcɛRIα; (Biolegend), BDCA1/CD1c, NKp44/CD336 (Beckman Coulter); BDCA2/CD303/CLEC4C, BDCA3/CD141, BDCA4/CD304 (Miltenyi Biotec); DCIR/CD367/CLEC4A (R&D systems), and FcɣRIIA/CD32, ILT7/CD85g (eBiosciences) antibodies. .. The analysis of CLRs expression after HBsAg exposure was performed using LSRII Flow Cytometer and FACSDiva software (BD).

Article Title: Characterization of four conventional dendritic cell subsets in human skin-draining lymph nodes in relation to T-cell activation.
Article Snippet: .. The imprints and cytospins were airdried overnight, fixed in acetone for 10 minutes, and stained by the use of a biotin-streptavidin staining technique as described previously.28 CD1a, BDCA3/CD141 (Miltenyi Biotec), CD14 (Novocastra), DC-SIGN (BD), and CD3 mAbs (Dakopatts) were used at dilutions of 1:10. .. Cytospins were made of the FACS-sorted cDC subsets and subsequently stained according to May Grünwald-Giemsa.28 At room temperature, with air as medium, expression was analyzed by the use of a Leica DMR microscope (Leica) with 40 /0.75 HCX PL Fluotar and 10 /0.30 and 20 /0.50 HC PL Fluotar objectives (Leica).

Flow Cytometry:

Article Title: Molecular and Functional Characterization of Lymphoid Progenitor Subsets Reveals a Bipartite Architecture of Human Lymphopoiesis.
Article Snippet: .. Cells were allowed to differentiate for 7 days before flow cytometry with a BD Fortessa Analyzer using the following antibodies: CD45 AF700 (Biolegend, clone HI30), CD14 PE-CF594 (BD bioscience, clone MOP9); CLEC9A APC (Biolegend, clone 8F9); CD66a,c,e (PE, Biolegend, clone ASL32); BDCA3/CD141 (PE-Vio770 Miltenyi, clone AD5-14H12); BDCA1/CD1c FITC (Miltenyi, clone AD5-8E7); CD123 BV786 (BD Bioscience, 7G3). .. Cells were allowed to differentiate for 7 days before flow cytometry with a BD Fortessa Analyzer using the following antibodies: CD45 AF700 (Biolegend, clone HI30), CD14 PE-CF594 (BD bioscience, clone MOP9); CLEC9A APC (Biolegend, clone 8F9); CD66a,c,e (PE, Biolegend, clone ASL32); BDCA3/CD141 (PE-Vio770 Miltenyi, clone AD5-14H12); BDCA1/CD1c FITC (Miltenyi, clone AD5-8E7); CD123 BV786 (BD Bioscience, 7G3).

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